Journal: Journal of Functional Biomaterials

Article Title: Differential Expression of S100A Genes in hDPSCs Following Stimulation with Two Hydraulic Calcium Silicate Cements: A Laboratory Investigation

doi: 10.3390/jfb17010055

Figure Lengend Snippet: Cell characterization of human dental pulp stem cells (hDPSCs). Immunofluorescence staining of hDPSCs revealed positive expression for Stro-1 ( a ), Nestin ( b ), and CD44 ( c ). Vizualization of various differentiation and staining protocols ( d – i ). Adipocyte differentiation was verified by Oil red O staining showing characteristic lipid packed vacuoles in the cytoplasm (red) ( e ) compared to undifferentiated control cells ( d ). Osteoblastic-like ( f ) and odontoblast-like ( g ) phenotype was affirmed by Alizarin Red (( f ); upper well: control cells, lower well: stimulated cells) and DSPP staining ( g ). Chondrocyte differentiation was confirmed by Toluidin blue (( h ); glycosaminoglycans are stained blue) and Collagen II (red) staining ( i ). White scale bars indicate 100 µm, whereas the red scale bar indicates 500 µm.

Article Snippet: After one week, a sufficient number of cells had migrated from the pulpal tissue, and STRO-1–expressing cells were enriched by magnetic-activated cell sorting (MACS ® ; Miltenyi Biotec, Bergisch Gladbach, Germany) using a mouse monoclonal antibody against STRO-1 (R&D Systems) in accordance with the manufacturer’s protocol.

Techniques: Cell Characterization, Immunofluorescence, Staining, Expressing, Control

Journal: Journal of endodontics

Article Title: Cells Isolated from Inflamed Periapical Tissue Express Mesenchymal Stem Cell Markers and Are Highly Osteogenic

doi: 10.1016/j.joen.2011.05.022

Figure Lengend Snippet: Immunohistochemical staining of inflamed periapical tissues. Representative data from 1 sample indicate positive staining of STRO-1 (A), CD90 (B), and CD146 (C) (arrowheads). (D) Nonimmune control. Scale bar: 100 μm (A–D).

Article Snippet: Antibodies Primary antibodies used for immunohistochemistry were mouse immunoglobulin G (IgG) control, mouse monoclonal anti-human STRO-1 (Invitrogen Corporation, Carlsbad, CA), mouse monoclonal anti-human CD90 (BD Biosciences, San Jose, CA), and mouse monoclonal anti-MUC18 (CD146) (Invitrogen Corporation).

Techniques: Immunohistochemical staining, Staining